EXPERIMENTAL EVIDENCE OF VENTRICULAR ARRHYTHMIAS SUSCEPTIBILITY IN DIFFERENT ANIMAL MODELS
Tématický okruh: Poruchy rytmu, kardiostimulace | |
| Typ: Ústní sdělení - lékařské , Číslo v programu: 213 | |
| Přihlášeno do: Soutěž mladých kardiologů | |
| Wojtaszczyk A.1, Stárek Z.2, Pešl M.3, Caluori G.4, Lehar F.1, Jež J.1, Wolf J.1, Crha M.5, DeSimone C.6, Asirvatham S.7 1 Cardioangiology and ICRC, University Hospital U Sv. Anny FNUSA-ICRC, Brno, 2 Cardioangiology and ICRC, 3 Cardioangiology and ICRC, University Hospital U Sv. Anny FNUSA-ICRC, and Masaryk University, Medical Faculty, Brno, 4 University Hospital U Sv. Anny FNUSA-ICRC, Cardioangiology and ICRC, Brno, 5 Fakulta veterinárního lékařství, Veterinární a farmaceutická univerzita, Brno, 6 Mayo Clinic Ventures, Mayo Clinic, Rochester, MN, United States, 7 Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, United States | |
Aim Among the animal models used in preclinical cardiology, the porcine one is the most accepted. It has widely replaced the canine model because of its similarity to the human heart anatomy and the ethical restrictions. Anatomical differences of the pig conductive system, with respect to the canine and human ones, are considered to be the cause of important electrophysiological properties, such as the susceptibility to ventricular arrhythmias (VAs). Material and Methods We analyzed a group of 6 pigs (FNUSA-ICRC, Veterinary and Pharmaceutical University /VFU/ Brno, CZ) and 6 mongrel dogs (Mayo Clinic, Rochester, USA) in which we performed endocardial and epicardial radiofrequency ablation (RFA, in total 62 and 41 applications respectively), while reporting the occurrence of spontaneous arrhythmias during the procedures. Other analyzed parameters were ablation power, duration, site (right and left ventricle) and device type (fork and finger catheter). A quasi binomial variance was assumed for absence or presence of VAs over the RFAs. Experiment was approved by Ethical Committee of VFU. Results Mean power and duration of Radiofrequency ablation (RFA) application when arrhythmia was not elicited were 36.52 ± 2.35 W, 111.25 ± 5.03 s and 31.69 ± 2.36 W, 49.16 ± 5.05 s (for dogs and pigs, respectively). Odds ratio (OR) estimated for porcine model showed almost five-fold susceptibility of VAs than canine one during RFA (OR 4.8201 vs. 1.5728; p=0.0101). Results were solely imputable to the investigated species, not to other ablation parameters. Conclusion Our work has shown that pigs are significantly more susceptible to ventricular arrhythmias than the dogs. This fact may limit the application of porcine model and should be taken under consideration during experiments designing. | |
